منابع مشابه
Multiplex DNA typing of short-tandem-repeat loci on the Y chromosome.
To facilitate evolutionary and forensic studies of DNA polymorphisms on the Y chromosome, we devised a multiplex amplification procedure for short-tandem-repeat (STR) loci. Four tetranucleotide STR loci (DYS19, DYS390, DYS391, and DYS393) were simultaneously amplified with FAM-labeled primers and genotypes were determined with an automated DNA sequencer. We typed 162 males from three U.S. popul...
متن کاملValidation of highly polymorphic fluorescent multiplex short tandem repeat systems using two generations of DNA sequencers.
Validation studies are a crucial requirement before implementation of new genetic typing systems for clinical diagnostics or forensic identity. Two different fluorescence-based multiplex DNA profiling systems composed of amelogenin, HumD21S11 and HumFGA (referred to as multiplex 1A), and HumD3S1358, HumD21S11 and HumFGA (multiplex 1B) have been evaluated for use in forensic identification using...
متن کاملAutomated DNA profiling employing multiplex amplification of short tandem repeat loci.
We have employed automated fluorescence-based technology to detect amplified tri-, tetra-, and pentanucleotide short tandem repeat (STR) loci electrophoresed on denaturing polyacrylamide sequencing gels. The system described incorporates an internal size standard in each sample, allowing the STR-PCR products to be sized automatically with a high degree of precision. By utilizing different fluor...
متن کاملAn Ultra-High Discrimination Y Chromosome Short Tandem Repeat Multiplex DNA Typing System
In forensic casework, Y chromosome short tandem repeat markers (Y-STRs) are often used to identify a male donor DNA profile in the presence of excess quantities of female DNA, such as is found in many sexual assault investigations. Commercially available Y-STR multiplexes incorporating 12-17 loci are currently used in forensic casework (Promega's PowerPlex Y and Applied Biosystems' AmpFlSTR Yfi...
متن کاملEvaluation of two short tandem repeat multiplex systems for post-haematopoietic stem cell transplantation chimerism analysis.
INTRODUCTION The follow-up of chimerism status after allogeneic haematopoietic stem cell transplantation (HSCT) is essential to predict successful engraftment to assess the development of graft-versus-host disease, graft rejection and disease relapse. Analysis of short tandem repeats (STR) via polymerase chain reaction is frequently used for chimerism determination. However, most commercially-a...
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ژورنال
عنوان ژورنال: American Journal of Clinical Pathology
سال: 2003
ISSN: 0002-9173,1943-7722
DOI: 10.1309/mgw2xkft0nyqcuhw